Ahmad Saleem Akhtar - ESR7
DVD based molecular diagnostic platform for UTI
Point of care tests for urinary tract infections aimed for primary care should be very simple and cost-effective. In this project, ESR 7 will be working on the development of DVD-based POC diagnostic platform for UTIs. The system will use centrifugal microfluidics for sample handling and processing and an optical DVD drive as Laser Scanning Microscope for detection. Towards this, ESR 7 will miniaturize and adapt molecular tools for adaptation of nucleic acid assays on the DVD platform in collaboration with ESRs 5 and 9. ESR 7 will in particularly be working on the following tasks:
Centrifugal microfluidics to manipulate urine samples; more specific on various filtration principles to isolate bacteria from urine sample for downstream processing
Bioassay development for nucleic acid extraction and processing on CD microfluidic platform; more specific bacteria lysis, DNA extraction, isothermal amplification and microarray based detection of amplified products.
Integrate the assay on DVD drive for hands-off processing of urine sample; more specific, develop a proof of concept 3-plex bioassay (E. coli, S. saprophyticus and Klebsiella spp.), based on isothermal amplification amended for use with centrifugal microfluidics
I want to use my technical knowledge and my interest in Biology to find solutions for medical problems. My aim is to develop a Point of Care (POC) system for rapid disease diagnostics that can be used in low resource settings. In particular, I am interested in the field of Centrifugal Microfluidics.
- National University of Science and Technology (NUST), Pakistan
BSc in Electrical Engineering, 2013
- Albert-Ludwigs-Universität Freiburg, Germany
MSc in Microsystems Engineering, 2017
- University of Antwerp – Vaccine & Infectious Disease Institute
Campus Drie Eiken – Universiteitsplein 1